Optical Signature Analysis of Liver Ablation Stages Exploiting Spatio-Spectral Imaging

Aref, Mohamed and Abdlaty, Ramy and Abbass, Mohamed and Aboughaleb, Ibrahim and Nassar, Ayman and Youssef, Abou-Bakr (2021) Optical Signature Analysis of Liver Ablation Stages Exploiting Spatio-Spectral Imaging. Journal of Biomedical Photonics & Engineering, 7 (2). 020306. ISSN 24112844

[thumbnail of 3412-9935-1-PB.pdf] Text
3412-9935-1-PB.pdf - Published Version

Download (12MB)

Abstract

Background and Objective: Thermal ablation modalities such as Radiofrequency ablation (RFA) / Microwave ablation (MWA) are deliberately used for marginally invasive tumor removal by escalating tissue temperature. For precise tumor extinguish, thermal ablation outcomes need routine monitoring for tissue necrosis in a challenging research task. The study aims to exploit hyperspectral imaging (HSI) to evaluate the impact of the liver tissue ablation. Materials and Methods: RFA with temperature range (≥80 °C) was accomplished on the ex vivo animal liver and evaluated using a spectral camera (400~1000 nm). The spectral signatures were extracted from the HSI data after the following processing steps: capturing three spectral data cubes for each liver sample with total 7-samples (before ablation, after ablation, and after ablation with sample slicing) using an HSI optical configuration. The custom HSI processing comprises “Top-hat and Bottom-hat transform” combined with “watershed transform” image segmentation to increase the intensity for a region of interest (ROI) of the investigated tissue, linking spectral and spatial data. Additionally, statistical analysis for HSI data was performed to exclusively select the best spectral band that discriminates between the normal, thermally-damaged, and ablated liver regions. Results: The variation of the optical parameters for the investigated liver samples provides variable interaction with the light diffuse reflection (Ŗd) over the spectrum range (400~1000 nm). Where, the extracting spectral information of the various tissue zones from the induced RFA linked to the hemoglobin, methemoglobin, and water permits variations. The generated spectral image after image enhancement utilizing “Top-hat and Bottom-hat transform” followed by “watershed segmentation”, showed high contrast between normal and thermal regions at a wavelength (600 nm). However, the wavelength (900 nm) shows a high variance between the normal and ablated regions. Finally, delineation of the thermal and ablated regions on the complemented enhanced image. Conclusion: HSI is considered a promising optical noninvasive technique for monitoring the RFA toward enhancing the ablation-based treatment for liver tumor outcomes.

Item Type: Article
Subjects: Bengali Archive > Multidisciplinary
Depositing User: Unnamed user with email support@bengaliarchive.com
Date Deposited: 14 Feb 2023 10:40
Last Modified: 14 Jun 2024 12:58
URI: http://science.archiveopenbook.com/id/eprint/256

Actions (login required)

View Item
View Item