Khan, Sana and Olszewski, Marie and Huang, Wei and Kletzel, Morris (2014) Juvenile myelo-monocytic leukemia (JMML): No effect of granulocyte monocyte-colony stimulating factor (GM-CSF) on Wilms Tumor gene (WT1) by nested Polymerase Chain Reaction (nPCR) and flow cytometry. Advances in Bioscience and Biotechnology, 05 (02). pp. 155-159. ISSN 2156-8456
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Abstract
This study was to determine whether GM-CSF induced WT1 gene expression and to establish an association with markers of proliferation CD71+CD34+ using nPCR and flow cytometry respectively, in samples obtained from 5 newly diagnosed JMML patients. Overtime (day 0 to day 14) there was an insignificant difference in WT1 gene expression and CD71+CD34+ in JMML samples when compared to peripheral blood of normal volunteers (n = 3). Our study suggests that there is a correlation between WT1 gene expression and cellular proliferation and that GMCSF in vitro does not create a significant difference in JMML samples.
Item Type: | Article |
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Subjects: | Bengali Archive > Biological Science |
Depositing User: | Unnamed user with email support@bengaliarchive.com |
Date Deposited: | 08 Mar 2023 12:09 |
Last Modified: | 17 Jun 2024 07:16 |
URI: | http://science.archiveopenbook.com/id/eprint/431 |